Laminar-Specific Vm Dynamics of L2/3 and L5 Neurons during Forepaw Behavior
(A) Example whole-cell recordings from a L2/3 neuron (red) and L5 neuron (blue) with the digit movement (green) measured by the stimulator/sensing arm in contact with the glabrous skin of forepaw digit 3.
(B) Population average fast Fourier transforms (FFTs) of the Vm of L2/3 and L5 neurons during quiet wakefulness (black) and digit movement (orange).
(C) Power of low-frequency activity (1–5 Hz) in the quiet (Q) and moving (M) state shows a significant reduction during digit movement in both L2/3 and L5 neurons. Filled circles with error bars show mean ± SEM, and lines represent individual neurons.
(D) SD of the Vm was significantly reduced during forepaw movement in both L2/3 and L5 neurons.
(E) L5 neurons showed an overall higher AP firing rate than L2/3 neurons in quiet and moving mice and a significant increase in AP firing rate during movement.
(F) AP threshold was not significantly different at rest and during movement in L2/3 and L5 neurons.
(G) Both L2/3 and L5 neurons depolarize during digit movement, but L2/3 neurons are more hyperpolarized than L5 neurons in both behavioral states.
(H) The mean firing rate (Q and M periods) of L2/3 and L5 neurons plotted as a function of the distance between AP threshold and the mean value of the maximum 10% of the Vm (Max Vm). Filled circles show the mean value for one cell.
For all panels, ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001. See also Figures S1–S3.