Figure 2.

Laminar-Specific V_m Dynamics of L2/3 and L5 Neurons during Forepaw Behavior

(A) Example whole-cell recordings from a L2/3 neuron (red) and L5 neuron (blue) with the digit movement (green) measured by the stimulator/sensing arm in contact with the glabrous skin of forepaw digit 3.

(B) Population average fast Fourier transforms (FFTs) of the V_m of L2/3 and L5 neurons during quiet wakefulness (black) and digit movement (orange).

(C) Power of low-frequency activity (1–5 Hz) in the quiet (Q) and moving (M) state shows a significant reduction during digit movement in both L2/3 and L5 neurons. Filled circles with error bars show mean ± SEM, and lines represent individual neurons.

(D) SD of the V_m was significantly reduced during forepaw movement in both L2/3 and L5 neurons.

(E) L5 neurons showed an overall higher AP firing rate than L2/3 neurons in quiet and moving mice and a significant increase in AP firing rate during movement.

(F) AP threshold was not significantly different at rest and during movement in L2/3 and L5 neurons.

(G) Both L2/3 and L5 neurons depolarize during digit movement, but L2/3 neurons are more hyperpolarized than L5 neurons in both behavioral states.

(H) The mean firing rate (Q and M periods) of L2/3 and L5 neurons plotted as a function of the distance between AP threshold and the mean value of the maximum 10% of the V_m (Max V_m). Filled circles show the mean value for one cell.

For all panels, ^∗p < 0.05, ^∗∗p < 0.01, and ^∗∗∗p < 0.001. See also Figures S1–S3.