Figure 6.

Tactile-Evoked Subthreshold Responses Are Highly Correlated across Cortical Layers and Modulated by Behavioral State

(A) Single-trial tactile-evoked responses from a dual L2/3 (red) and L5 (blue) whole-cell recording during quiet wakefulness (left) and movement (right).

(B) Top: mean V_m tactile-evoked response of example neurons in (A) during quiet wakefulness and movement shows reduction in amplitude during moving periods. Bottom: corresponding PSTH of AP firing.

(C) Population averaged subthreshold tactile-evoked response during quiet wakefulness and movement.

(D) Population PSTH from L2/3 and L5 to tactile stimulation during quiet wakefulness and movement.

(E) L2/3 and L5 neurons show a decrease in tactile-evoked subthreshold response amplitude as mice go from quiet (Q) to movement (M). Filled circles with error bars show mean ± SEM. Lines show individual cells.

(F) Population analysis of background-subtracted AP firing rates to tactile stimulation of the forepaw shows no difference between Q (left) and M (right) periods in both layers. AP rate measured as the difference between the 100 ms before and 100 ms after stimulus onset.

(G) The subthreshold tactile-evoked reversal potential shows no significant difference between layers or behavioral states.

(H) Absolute AP rate in the 100 ms after stimulus onset plotted as a function of the difference in V_m between AP threshold and the tactile stimulus-evoked response reversal potential. Filled circles show mean value of individual cells.

(I) Subthreshold tactile-evoked response latencies of L2/3 and L5 neurons are not significantly different.

(J) Amplitude of L2/3 subthreshold tactile-evoked responses plotted against the amplitude of L5 subthreshold responses from the example pair in (A) shows highly correlated response amplitudes during Q (black) and M (orange) periods.

(K) Population data of cross-correlation of mean subthreshold tactile-evoked responses (combining Q and M responses) between L2/3 and L5 neurons. Filled circles with error bars show mean ± SEM, and open circles show individual cells.

For all panels, ^∗p < 0.05, ^∗∗p < 0.01, and ^∗∗∗p < 0.001. See also Figures S6 and S7.