FIG. 5.

Pretreatment with MH (10 μM) blocked nicotine-induced PERK phosphorylation after 6 h in Rcho-1 TG cells. A, Specific targeted protein bands as detected by respective antibodies via Western blot. B, Protein levels of the ratio of P-PERK[Thr981]: PERK at 6 h. All arbitrary values were expressed as means normalized to Amido Black ± SEM. All experiments were performed in quadruplicates (n = 4). Significant differences between treatment groups as determined by 1-way ANOVA (1WA) indicated by * ( P < .05). Different letters represent means that are significantly different from one another according to Tukey’s posttest (P < .05).