FIG. 6.

The effect of nicotine exposure (1–10 μM) on markers of placental TG cell differentiation and function after 24 h in Rcho-1 TG cells. mRNA levels of A, Pl-1, B, Pgf, C, Hsd11b1, and D, Hsd11b2. All arbitrary values were expressed as means normalized to the geometric mean of β-Actin and Gapdh ± SEM. All experiments were performed in quadruplicates (n = 4). All experiments were performed in quadruplicates (n = 4). Significant differences between treatment groups as determined by 1-way ANOVA (1WA) indicated by * (P < .05). Different letters represent means that are significantly different from one another according to Tukey’s posttest (P < .05). Non-significant differences (P > .05) indicated by n.s.