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. 2016 Mar 22;151(1):10–22. doi: 10.1093/toxsci/kfw032

FIG. 2.

FIG. 2.

Validation of resistance to FA of select mutant clones. A, Viable cell density of lipoprotein receptor-related protein 5 (LRP5), glutamic-oxaloacetic transaminase 1 (GOT1), and meiosis 1 associated protein (M1AP) mutants and wild-type KBM7 cells, 72 h after treatment with FA. Average (and SD) of 3 replicate experiments shown as a percentage of untreated controls cells; B, Viable cell density of mitogen-activated protein kinase kinase 5 (MAP2K5), CTC1, and FCRLA mutants and wild-type KBM7 cells 72 h after treatment with FA. Average and SD of 2 replicate experiments shown as a percentage of untreated controls cells; C, Representative data (from 1 of 2 experiments, 2 replicates per experiment) showing live/dead LRP5, GOT1, and M1AP mutants and wild-type KBM7 cells, after treatment with FA (90 µM) for 72 h, compared with untreated cells; D, Validation of knockdown of LRP5 and M1AP mRNA in the mutant cells by RT-PCR normalized to the housekeeping gene ribosomal protein L13a. Data (fold change and SD) are averaged from 4 experiments and 3 experiments, respectively, 2 replicates per experiment; E, Validation of LRP5 protein knockdown in the mutant cells by Western blot. Representative data from 1 of 2 experiments shown. *P < .05 by Student’s t test.