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Cardiac-specific PDH E1α deficiency mice. A, Genotyping of CreERT2-PDHflox/flox and CreERT2(α-MHC-MerCreMer) mice by PCR analysis. B, Immunoblotting verified the level of PDH E1α in heart. The PDH E1α protein level was markedly reduced in the adult mouse heart 2 months after tamoxifen (TM) injection for 5 days. C, The immunoblotting shows the PDH E1α was specifically knock out in the adult mouse heart after TM injection.
