Figure 6.

Direct Tethering Function of NET39

(A) Schematic diagrams of NET39 and fusion constructs with FISH analysis and representative images of Ptn loci positioning in indicated samples. The error bars represent SD of the mean over two biological repeats. ^∗∗∗p < 0.001 and ^∗p < 0.05 comparing samples to NLS-GFP expressing MBs by χ² tests.

(B) Representative images and cumulative frequency distribution of Ptn loci distance from the edge of nucleoli in indicated samples expressing either the NET39 soluble fragment fused to nucleolin or nucleolin-GFP over two summed biological repeats. For all images, Ptn is labeled green and GFP is labeled red.

The scale bars represent 5 μm. See also Figure S7.