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. 2016 Jun 21;6:28329. doi: 10.1038/srep28329

Figure 3. Knocking down of EMMPRIN decreases breast cancer cells to show stem- like cell properties.

Figure 3

(A,B) EMMPRIN knocking down decreased the percentage of CD44+CD24− breast cancer cells. SUM102 and BT474 breast cancer cells were infected lentivirus mediated EMMPRIN knocking down, and the cells were treated with conditioned medium from Hs578Bst fibroblasts for 3 days, and then the cells were collected for mammosphere formation assay. The conditioned medium were collected from Hs578Bst fibroblasts or the breast cancer cells co-cultured with fibroblasts for 3–5 days. (C,D) Down-regulation of EMMPRIN decreased mammosphere formation in breast cancer cells. SUM102 and BT474 breast cancer cells with EMMPRIN knocking down or the control were treated with conditioned medium from 1068SK fibroblasts for 3 days and then the cells were digested and used for mammosphere formation assay. EMMPRIN knocking down induced changes of breast cancer stem cell markers. SUM102 (E,F) BT474 breast cancer cells were infected with LV-EMMPRIN shRNA, and the cells were treated with conditioned medium from fibroblasts 1068SK or Hs578Bst for 3 days, and then the protein were extracted from the cells for western blotting. The data presented were shown as means ± s.d. collected from three independent experiments. *p < 0.05, **p < 0.01.