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. 2016 Mar 25;95(7):742–751. doi: 10.1177/0022034516640246

Figure 2.

Figure 2.

Acellular cementum is not defective in Phospho1-/- mice. (A–F) Observation and histomorphometry of hematoxylin and eosin–stained histological sections of the first mandibular molar at 14, 30, and 90 d postnatal (dpn) reveals no defect in acellular cementum (AC) in Phospho1-/- compared with wild-type (WT) mice. (G, H) Histomorphometry of AC width indicates no deficit in Phospho1-/- mice compared with controls and a trend for increased AC thickness (*P < 0.05; ***P < 0.001). Osteopontin (OPN) immunostaining (insets in A, B) indicates no delay in AC initiation in Phospho1-/- versus WT molars at 14 dpn, confirmed by histomorphometric measurement in (I). Goldner’s trichrome staining (insets in C, D) performed on 30 dpn undecalcified samples suggests a similarly mineralized AC layer on Phospho1-/- and WT molar roots. Dentin (DE) defects are described in Appendix Figures 2 to 4. AB, alveolar bone; PDL, periodontal ligament.