Figure 5. Representative pyruvate dehydrogenase assay on cells.
Cells were treated with DCA, MCA and Pro-MCA-NP at 100 μM for 72 h before performing the assay, (A) comparative increase in PDH activity of cells correlated with increase in absorbance of reaction mixture at λ 450 nm where “red circle line” represents PDH activity in untreated cells and (B) time dependent increase in PDH activity of protein (125 μg/ml).