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. 2016 Jun 21;6:28437. doi: 10.1038/srep28437

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Each experiment was performed in triplicate (the error bar represents standard deviation). (a) Reaction velocity of the EphA2 mutants Tyr772Phe (black) and Arg762Ala/Tyr772Phe (red) are measured at increasing Mg²⁺ concentration in the range of 0–10 mM. (b) Reaction velocity of the PKA mutants Thr197Ala (black) and Lys189Ala/Thr197Ala (red) are measured in a similar manner to EphA2. (c) Reaction velocity of the p38MAPK mutants Thr180Ala (black) and Arg173Ala/Thr180Ala (red) are measured at Mg²⁺ concentrations in the range of 0–2 mM.