Figure 2.

The main zinc ion binding site is NRP2‐specific. (A) Stereoimage of the NRP2 residues involved in Zn²⁺ ion coordination: Y375, H377 and H399. The residues are shown using stick representation within the final 2 F _o – F _c electron density map contoured at 1 σ (grey mesh). (B) Sequence alignment for the part of the neuropilin sequences that includes all three residues that bind zinc in site ‘1’ shows that the site is conserved in NRP2 and absent in NRP1. The residues associated with NRP2 are highlighted in yellow, and the residues in the analogous positions in NRP1 are highlighted in green.