Effects on serum FSH during infusions of vehicle and SC kisspeptin‐54 in healthy women during the early follicular phase of the menstrual cycle. Mean serum FSH was collated from all participants after an 8‐h infusion of either vehicle or SC kisspeptin‐54 at 0·1, 0·3 and 1·0 nmol/kg/h doses. (a) shows the mean serum FSH results for each time point (from all 4 participants) during the 8‐h study presented as a time profile. (b) is a graphical representation of the mean serum FSH over the study period shown in (a). Black line and bar, vehicle; green line and bar, kisspeptin‐54 0·1 nmol/kg/h; blue line and bar, kisspeptin‐54 0·3 nmol/kg/hr; red line and bar, kisspeptin‐54 1·0 nmol/kg/h. Data are mean ± SEM. N = 4/group. Green *, P < 0·05 for SC 0·1 nmol/kg/h kisspeptin‐54 vs vehicle; blue ***, P < 0·001 for SC kisspeptin‐54 0·3 nmol/kg/h vs vehicle; red ***, P < 0·001 for SC kisspeptin‐54 1·0 nmol/kg/h vs vehicle.