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. 2016 Jun 21;6:28211. doi: 10.1038/srep28211

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Taq polymerase stalling assay in presence of LiCl, NaCl, KCl or (NH₄)₂SO₄ with control (CT) vs mutated (MCT) oligos using biotinylated primers (Primer). Amplified products were loaded on 15% denaturating urea-PAGE transferred to biodyne B membrane and blotted with avidin-HRP. Arrowheads indicate full-length product, FLP; truncated products, TP and biotinylated-primer, Primer. All the gels were run under the same experimental conditions and blots are presented by using cropped images.