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. 2016 Jun 21;6:28211. doi: 10.1038/srep28211

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Electrophoretic mobility shift assay was performed with biotinylated oligos forming G-quadruplex structures and rice nuclear proteins (5 and 10 μg) in the presence of different ions (NaCl and KCl). Protein-DNA complexes were resolved on 6% native acrylamide gel, transferred to biodyne B membrane and blotted with avidin-HRP. All the gels were run under the same experimental conditions and blots are presented by using cropped images.