Table 2. Oligo sequences used for GQS validation.
| Oligo ID | Sequence (5′-3′) | Molecularity | Structural characteristics |
|---|---|---|---|
| Os3 | GGGAAGAGGGAAGAGGGGAAGAGGGG | Intramolecular (KCl) | (3+1) and parallel (KCl): Positive peak at 290 and 260 nm |
| Os4 | GGGGGTTGGGGGAGGGTGGGGAAAGTCGGGG | Intramolecular (NaCl) | Anti-parallel (NaCl): Positive peak at 295 nm and negative peak 260 nm |
| Os6 | GGGAGGAGGGAGAAGGGTGGG | Intermolecular (KCl, NaCl): bimolecular and tetramolecular) | Parallel (NaCl): Positive peak at 260 nm (3+1) and parallel (KCl): Positive peak at 290 and 260 nm |
| Os9 | GGGCGCGAGGGAGGAGGGCGCGGG | Intramolecular (KCl, NaCl) | (3+1) and parallel (KCl, NaCl): Positive peak at 290 and 260 nm |
| Os11 | GGGACACGGGGGAGAACTTGGGCATGGGGAGGGTGGGCAGGG | Intramolecular (KCl) | Parallel (KCl): Positive peak at 260 nm |
| Os5 | GGGTCCTAGGGGTGGGGTGGGAAGGGTGGGAGGGGAAGGGGGAGGAGGGA | Intramolecular (KCl) | Parallel (KCl): Positive peak at 260 nm |
| Os7 | GGGTGTGGGGAGGGTGGGG | Intramolecular (KCl) | Parallel (KCl): Positive peak at 260 nm |
| Os10 | GGGGAGGAGGGAGGGTGGGTAGGGGGGGGAGGG | Intramolecular (KCl) | Parallel (KCl): Positive peak at 260 nm |
| Os13 | GGGGAGAGGGAGAAGGGGGAGGAGAAGGGAGAAGGGAGGG | Intramolecular (KCl, NaCl) | Parallel (KCl): Positive peak at 260 nm |
| Os18 | GGGGGGAGGGTGGGGAGTAGGG | Intramolecular (KCl, NaCl) | Parallel (KCl): Positive peak at 260 nm (3+1) and parallel (NaCl): Positive peak at 290 and 260 nm |
| At3 | GGGTGGCGGGAAAATTGGGGACTTAGGG | Intramolecular (KCl) | Parallel (KCl): Positive peak at 260 nm (3+1) and parallel (NaCl): Positive peak at 290 and 260 nm |
| At8 | GGGACGGGTTTGGCGGGACGGG | Intramolecular (NaCl) | (3+1) and parallel (NaCl): Positive peak at 290 and 260 nm |
| At12 | GGGTTGGTTGGATGG | Intermolecular (NaCl) | (3+1) and parallel (KCl, NaCl): Positive peak at 290 and 260 nm |
| At15 | GGTTTGGTTAGGGAGGG | Intramolecular (KCl) Intermolecular (NaCl) | (3+1) and parallel (KCl): Positive peak at 290 and 260 nm Anti-parallel (NaCl): Positive peak at 295 nm and negative peak 260 nm |
| At17 | GGTGGCGTGGCGG | Intramolecular (KCl) Intermolecular (NaCl) | (3+1) and parallel (KCl): Positive peak at 290 and 260 nm Anti-parallel (NaCl): Positive peak at 295 nm and negative peak 260 nm |
| Ca1 | GGGAGAAGGGAGAAGGGAGAAGGGAGAAGGG | Intramolecular (KCl, NaCl) | Parallel (KCl, NaCl): Positive peak at 260 nm |
| Ca2 | GGGGTGGGTGGGTAAGGTGGGG | Intermolecular (KCl, NaCl) | Parallel (KCl): Positive peak at 260 nm (3+1) and parallel (NaCl): Positive peak at 290 and 260 nm |
| Gm1 | GGGAGAAGGGAGAAGGGATGGGGTGGG | Intramolecular (KCl, NaCl) | Parallel (KCl, NaCl): Positive peak at 260 nm |
| Gm2 | GGGAGAAGGGAGAAGGGAAGGG | Intermolecular (KCl, NaCl) | Parallel (KCl): Positive peak at 260 nm (3+1) and parallel (NaCl): Positive peak at 290 and 260 nm |
| Gm4 | GGGTGGGGTTGGGAAGGTGGGAGGAGAGGGTGAGGG | Intramolecular (KCl) Intermolecular (NaCl) | Parallel (KCl): Positive peak at 260 nm Parallel (NaCl): Positive peak at 260 nm |
Os: Oryza sativa; At: Arabidopsis thaliana; Gm: Glycine max; Ca: Cicer arietinum.