Table 3. Oligo sequences containing GQSes used for polymerase stalling.

Oligo ID	Sequence (5′-3′)
Os3_CT	TCCAACTATGTATACTGAAGGGGAAGAGGGAAGAGGGGAAGAGGGGAAAATTAGCAACACGCAATTGCTATAGTGAGTCGTATTA
Os3_MCT	TCCAACTATGTATACTGAAGGGGAAGAGAGAAGAGGAGAAGAGAGGAAAATTAGCAACACGCAATTGCTATAGTGAGTCGTATTA
Os4_CT	TCCAACTATGTATACTGAAGGGGGGTTGGGGGAGGGTGGGGAAAGTCGGGGAAAATTAGCAACACGCAATTGCTATAGTGAGTCGTATTA
Os4_MCT	TCCAACTATGTATACTGAAGGGGGGTTGGAGGAGAGTGGAGAAAGTCGGGGAAAATTAGCAACACGCAATTGCTATAGTGAGTCGTATTA
Os6_CT	TCCAACTATGTATACTGAAGGGGAGGAGGGAGAAGGGTGGGAAAATTAGCAACACGCAATTGCTATAGTGAGTCGTATTA
Os6_MCT	TCCAACTATGTATACTGAAGGAGAGGAGAGAGAAGAGTGAGAAAATTAGCAACACGCAATTGCTATAGTGAGTCGTATTA
Os9_CT	TCCAACTATGTATACTGAAGGGGCGCGAGGGAGGAGGGCGCGGGAAAATTAGCAACACGCAATTGCTATAGTGAGTCGTATTA
Os9_MCT	TCCAACTATGTATACTGAAGGGACGCGAGAGAGGAGAGCGCGAGAAAATTAGCAACACGCAATTGCTATAGTGAGTCGTATTA
Os11_CT	TCCAACTATGTATACTGAAGGGGACACGGGGGAGAACTTGGGCATGGGGAGGGTGGGCAGGGAAAATTAGCAACACGCAATTGCTATAGTGAGTCGTATTA
Os11_MCT	TCCAACTATGTATACTGAAGGGGACACGGAGGAGAACTTGAGCATGGAGAGAGTGGGCAGGGAAAATTAGCAACACGCAATTGCTATAGTGAGTCGTATTA
Bio_Primer	[Biotin]TAATACGACTCACTATAGCAATTGCGTG

Sequences in bold and underline represent G residues predicted to be involved in GQS formation and their mutated counterparts in MCT oligos. CT: control oligos; MCT: mutated oligos.