Table 2. Clinical information for women whose NAF samples passed quality filtering.

		Healthy Control Women (HC)	Women with a History of Breast Cancer (BC)
Age	average (range)	52 (41 to 64)	58 (52 to 66)
Caucasian	n (%)	6 (100%)	6 (100%)
BMI	average ± SD (range)	25.6 ± 8.1 (19.5 to 35.1)	24.3 ± 4.6 (20.6 to 31.3)
Menopausal status	n (%)
Pre-menopausal		2 (33.3%)	1 (16.7%)
Peri-menopausal		1 (16.7%)	1 (16.7%)
Post-menopausal		3 (50.0%)	4 (66.6%)
Estrogen Receptor (ER)	n (%)
Positive		NA	3 (50.0%)
Negative		NA	3 (50.0%)
Progesterone Receptor (PR)	n (%)
Positive		NA	1 (16.7%)
Negative		NA	2 (33.3%)
Unknown		NA	3 (50.0%)
Human Epidermal Growth Factor Receptor 2 (HER2)	n (%)
Positive		NA	3 (50.0%)
Negative		NA	3 (50.0%)
Breast Feed	n (%)
Yes		2 (33.3%)	3 (50.0%)
No		4 (66.7%)	2 (33.3%)
Unknown		0 (00.0%)	1 (16.7%)
Antibiotics in the Past Year	n (%)
Yes		3 (50.0%)	1 (16.6%)
No		3 (50.0%)	4 (66.7%)
Unknown		0 (00.0%)	1 (16.7%)
Hormone therapy/Oral contraceptive	n (%)
Yes		3 (50.0%)	2 (33.3%)
No		3 (50.0%)	3 (50.0%)
Unknown		0 (00.0%)	1 (16.7%)
Recent Breast Exposure to Mouth by Breast-feeding/Sex	n (%)
Yes		3 (50.0%)	3 (50.0%)
No		3 (50.0%)	2 (33.3%)
Unknown		0 (00.0%)	1 (16.7%)
Mother had Breast Cancer	n (%)
Yes		2 (33.3%)	1 (16.7%)
No		4 (66.7%)	4 (66.7%)
Unknown		0 (00.0%)	1 (16.7%)
Dense Breast Tissue on Mammogram	n (%)
Yes		4 (66.7%)	3 (50.0%)
No		2 (33.3%)	1 (16.7%)
Unknown		0 (00.0%)	2 (33.3%)

Demographics for 6 healthy control women and 6 women with a history of breast cancer whose NAF samples had sufficient reads remaining after quality filtering and background subtraction removing all OTUs detected in the empty control Eppendorf tube.