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. 2016 Jun 21;6:28132. doi: 10.1038/srep28132

Figure 1. PKA-CQR expression alone is sufficient to activate CREB, β-catenin and GSK-3β signaling in undifferentiated GCs.

Figure 1

Undifferentiated GCs were isolated, transduced with control EGFP or PKA-CQR mutant lentivirus vectors (2MOI), and stimulated with FSH as described in Methods. Whole cell protein extracts were prepared and run on SDS-PAGE gels. Separated proteins on gels were transferred and probed with antisera against PKAc, p-CREB (Ser133), CREB, p-β-catenin (Ser552), β-catenin, p-GSK-3β (Ser9) and GSK-3β as described in Methods (panel A). The densitometric quantification of the immunoblot results are shown in panels (B–E). Asterisks indicate results that are statistically different from EGFP controls (P < 0.05, n = 3). This figure contains cropped panels of immunoreactive bands representing phosphorylated and total protein levels from the same protein extracts resolved in separate immunoblots. Vertical lines between individual bands indicate bands from the same immunoblots that were rearranged for clarity of presentation. Full length blots are presented in Supplementary Fig. 1.