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. 2016 Jun 21;6:28359. doi: 10.1038/srep28359

Table 1. EC50 and contact distances for cadaverine and putrescine amino groups with Asp1123.32 and Asp2025.42 for mutant and wildtype TAAR13c.

Wildtype/Mutant EC50 (μM, mean+/−SEM)
Distance to Cad-NH2
Distance to Put-NH2
cad put pos112 pos202 pos112 pos202
TAAR13c 15.2 ± 2.2 >1000 2.7 3.2 3.0 5.5
D112A loss loss 2.4 10.8 10.7 4.9
D112E ≫1000 loss 9.2 3.2 9.8 5.0
D112N loss loss 12.0 3.1 12.0 3.1
D202A loss loss 2.4 10.8 10.7 4.9
D202E loss loss 9.2 3.2 9.8 5.0
D202N loss loss 3.0 9.5 2.5 10.6
D279A 2.1 ± 1.0 31 ± 13 3.0 3.7 3.0 6.4
D279E 0.72 ± 0.24 10 ± 4 2.9 3.5 3.0 5.6
D279N 1.1 ± 0.8 7.3 ± 3.6 2.9 3.5 3.0 5.6
W296F 42 ± 6 >1000
W296G 55 ± 48 loss
W296Y 28 ± 8 >1000
D78E 64 ± 28 >1000