Table 2. Sequences of mutagenic primers used.

Wildtype/Mutant	Forward Primer, 5′-3′orientation	Reverse Primer, 5′-3′ orientation
TAAR13c	atggatttatcatcacaagaa	tcaaaccgtaaataaattgat
D112A	ccggttttgccctgtttctcac	gtgagaaacagggcaaaaccgg
D112N	acaccggttttaacctgtttctcac	gtgagaaacaggttaaaaccggtgt
D112E	ccggttttgaactgtttctcac	gtgagaaacagttcaaaaccgg
D202A	tggtcagttttagccacattacta	tagtaatgtggctaaaactgacca
D202N	tggtcagttttaaacacattacta	tagtaatgtgtttaaaactgacca
D202E	tggtcagttttagagacattacta	tagtaatgtctctaaaactgacca
D279A	actctctggtggctccctacattaac	gttaatgtagggagccaccagagagt
D279N	actctctggtgaatccctacattaac	gttaatgtagggattcaccagagagt
D279E	actctctggtggagccctacattaac	gttaatgtagggctccaccagagagt
W296G	tgatgcatttggtgggttaggctacac	gtgtagcctaacccaccaaatgcatca
W296Y	tgatgcatttggttacttaggctacac	gtgtagcctaagtaaccaaatgcatca
W296F	tgatgcatttggtttcttaggctacac	gtgtagcctaagaaaccaaatgcatca
D78E	ctggctctggcggaactgctgg	ccagcagttccgccagagccag