Figure 5. KRT14^pos cells show increased in vitro clonogenic capacity.

(a) Tomato^neg (Tom^neg) and Tomato^pos (Tom^pos) cells from tamoxifen-injected 8-week-old mice, were FACS sorted with Tomato positivity using a Becton Dickinson FACS Aria IIu cell sorter, and cultured in Matrigel. Cultures at 11 days are shown. (b) High magnification of a Tom^pos sphere from a. (c) Sphere-forming efficiency of Tom^pos (9.21±0.61%, n=10) and Tom^neg (0.56±0.09%, n=10) urothelial cells from a. (d) IF against KRT5 and KRT14 of Matrigel-grown Tom^pos spheres indicating stratification. (e) Sphere forming efficiency of passaged (P1) Tom^pos (50.88±2.37%, n=10) and Tom^neg (16.04±0.94%, n=9) urothelial cells obtained from Matrigel cultures from a. For c and e, two group comparison was performed using the Student's t-test. **P<0.0001. Scale bars represent 1 mm in a, and 100 μm in b and d.