Fig. 2.

TRIM11 was highly expressed in lung cancer cells and its expression was suppressed by TRIM11 siRNA transfection. a Real-time PCR analysis for the TRIM11 mRNA expression level in 5 lung cancer cell lines (A549, NCI-H446, NCI-H1975, NCI-H460 and PC-9) and 1 human embryo lung fibroblast cell line (MRC-5). **P < 0.01 and ***P < 0.001 versus MRC-5 cells. b TRIM11 protein expression was determined by Western blot. Quantification (right panel) based on at least 3 independent experiments was made by normalizing against GAPDH levels. **P < 0.01 and ***P < 0.001 versus MRC-5 cells. c Real-time PCR analysis showed the efficiency of TRIM11 knockdown in A549 and NCI-H446 cells. WT: wild-type cells. NC: control siRNA-transfected cells. siRNA1, 2 and 3: TRIM11 siRNA1, 2 or 3-transfected cells. ***P < 0.001 versus NC. d The protein levels of TRIM11 were determined by Western blot and GAPDH was used as an internal control. Quantification (right panel) was shown. ***P < 0.001 versus NC