Figure 4.
(A and B) Osteocyte apoptosis and RANKL expression in fatigued mouse ulnae treated in vivo with the P2X7R antagonist, Brilliant Blue G (BBG) or Vehicle (VEH). BBG inhibited the normal increase in osteocyte RANKL expression in ulnar cortex following fatigue loading (p<0.05), but did not alter the amount of osteocyte apoptosis. (C and D) BBG treatment dramatically reduced the activation of resorption after fatigue at both intracortical (Rs.N, p<0.06 vs VEH) and endocortical (En.Tun.N, p<0.05 vs VEH) locations.