Macrophage-stimulated invasion of (A) MDA-MB-231 or (B) BT-549 cells is expressed as the fold change in the number of invaded cells, relative to values obtained with unstimulated cells expressing wild type p110β. Macrophage-stimulated invasion of parental (C) MDA-MB-231 or (D) BT-549 cells without or with treatment with TGX-221 is expressed as the fold change in the number of invaded cells, relative to values in the absence of macrophages and TGX-221. E) Macrophage-stimulated invasion of MDA-MB-231 cells in a 3D in vitro collagen invasion assay is expressed as the fraction of cells that invaded ≥ 20 µm. F) Invasion of macrophages, co-plated with MDA-MB-231 cells in the 3D invasion assay is expressed as the number of cells that invaded ≥ 20 µm. Data represent the mean ± SEM from three (A, B, C, E, and F) or two (D) independent experiments. *: p<0.05; **: p<0.01; ***: p<0.001; ****: p<0.0001