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. Author manuscript; available in PMC: 2017 May 15.
Published in final edited form as: Cancer Res. 2016 Mar 24;76(10):2944–2953. doi: 10.1158/0008-5472.CAN-15-1675

Figure 3. Gβγ binding to p110β is required for macrophage-stimulated breast cancer cell invasion.

Figure 3

Macrophage-stimulated invasion of (A) MDA-MB-231 or (B) BT-549 cells is expressed as the fold change in the number of invaded cells, relative to values obtained with unstimulated cells expressing wild type p110β. Macrophage-stimulated invasion of parental (C) MDA-MB-231 or (D) BT-549 cells without or with treatment with TGX-221 is expressed as the fold change in the number of invaded cells, relative to values in the absence of macrophages and TGX-221. E) Macrophage-stimulated invasion of MDA-MB-231 cells in a 3D in vitro collagen invasion assay is expressed as the fraction of cells that invaded ≥ 20 µm. F) Invasion of macrophages, co-plated with MDA-MB-231 cells in the 3D invasion assay is expressed as the number of cells that invaded ≥ 20 µm. Data represent the mean ± SEM from three (A, B, C, E, and F) or two (D) independent experiments. *: p<0.05; **: p<0.01; ***: p<0.001; ****: p<0.0001