A) The number of metastases per lung section from SCID mice injected with MDA-MB-231 cells expressing wild type or mutant p110β into the lateral tail vein. B) Average size of lung metastases. The data represent the mean ± SEM from 4, 6, and 5 mice for wild type p110β, p110βKK-DD, or p110βK-R, respectively. Macrophage-stimulated transmigration of (C) MDA-MB-231 or (D) BT-549 cells expressing wild type or mutant p110β in the eTEM assay is expressed as the fold change in the number of transmigrated cells relative to values obtained from cells expressing wild type p110β. The data represent the mean ± SEM from three independent experiments. *: p<0.05; **: p<0.01; ***: p<0.001; ****: p<0.0001; ns: not significant.