Fig. 2. Kaiso represses GR expression in breast cancer cell lines. (A) Kaiso and GR expression levels in breast cancer cell lines were determined by western blotting. (B) Kaiso and GR expression levels were determined by western blotting in siRNA control or Kaiso-knockdown T47D cells. (C) Overexpression of Kaiso in breast cancer cell lines and GR and Kaiso expression levels were determined by western blotting. (D) Kaiso mRNA levels in vector control or Kaiso-overexpressing breast cancer cell lines were determined by real-time PCR. (E) Overexpression of Kaiso in MCF-7 cells decreased the luciferase activity of the GR promoter-reporter in a dose-dependent manner.
