Fig. 4. Kaiso attenuates the anti-apoptotic effect of GR signaling in breast cancer cells. (A) MDA-MB-231 cells were transfected with vector control or FLAG-Kaiso and then serum starved for 72 h in the presence or absence of dexamethasone (106 M). The percentage of apoptotic cells was determined by Annexin V staining. (B) MCF-7 cells were transfected with vector control or FLAG-Kaiso and then treated with TNF-α (10 ng/ml) for 48 h in the presence or absence of dexamethasone. The percentage of apoptotic cells was determined by Annexin V staining. (C) MCF-7 cells were transfected with vector control, FLAG-Kaiso or Myc-GR as indicated, and the expression levels of Kaiso and GR were determined by western blotting. (D) The cells used in (C) were treated with TNF-α (10 ng/ml) for 48 h in the presence or absence of dexamethasone. The percentage of apoptotic cells was determined by Annexin V staining. (E) p65 phosphorylation and Sgk-1 expression in the MCF-7 cells used in (C) were determined by western blotting. (F) ICAM-1 and cIAP2 expression levels in MCF-7 cells were determined by real-time PCR. Data shown represent the results of three independent experiments. **P ＜ 0.01.
