Fig. 3. Overexpression of miR-185 attenuates ERS-induced apoptosis. (A) Twenty four hours after transfection with miR-185 mimic (in parallel to a negative control(NC)), cardiomyocytes were exposed to 100 ng/ml TM for 48 h. Apoptotic cells represented by the TUNEL-positive cells (magenta in the merged images) were counted. Nuclei were identified by staining with Hoechst 33342 (blue). (B) Quantification of the percentage of TUNEL-positive cardiomyocytes. (C, D) Twenty-four hours after transfection with increasing concentrations of miR-185 mimic, cardiomyocytes were treated with 100 ng/ml TM for 24 h. Western blotting was performed using antibodies against CHOP and cleaved-caspase 3. α-tubulin is shown as a loading control. Results are presented as mean ± SEM; N = 3; Statistical significance is shown as *P ＜ 0.05 or **P ＜ 0.001. Data were statistically analyzed by one-way ANOVA followed by a Bonferroni’s multiple-comparison of multiple means.
