Fig. 3. Effects of DAB on HMGB1-mediated pro-inflammatory responses. (A-D) HUVECs were stimulated with HMGB1 (1 μg/ml) for 16 h, followed by treatment with DAB for 6 h. HMGB1-mediated (A) expression of VCAM-1 (white bar), ICAM-1 (gray bar), and E-selectin (black bar) in HUVECs, (B, C) adherence of human neutrphils to HUVEC monolayers, and (D) migration of human neutrphils through HUVEC monolayers were analyzed. (E) The effects of treatment with DAB on HMGB1-induced (2 μg/mouse, i.v., white box) or CLP-induced (black box) leukocyte migration into the peritoneal cavities of mice were analyzed. The representative images were from three separate experiments in different days with similar results. All results indicate the mean ± SEM of three separate experiments in different days. *P ＜ 0.05 vs. HMGB1.
