Fig. 1. SETDB1 is commonly regulated by various anticancer drugs. (A) A549 cells were treated with various anticancer drugs including taxol (0.5 μM), cisplatin (2 μM), 5-FU (5 μM), and doxorubicin (2 μM). SETDB1 protein was down-regulated, whereas p53 protein was up-regulated in these treatments. (B) Luciferase assay was performed following anticancer drug treatment after pGL3-SETDB1-p-Luc transfection. (C) Western blots were done following doxorubicin or taxol treatment. SETDB1 was decreased, p53 was increased, but EZH2 or SUV39H1 displayed no change in protein levels. (D) Doxorubicin was treated in a dose- or time-dependent manner in A549 cells. H3K9me3 levels decreased, consistent with SETDB1 down-regulation.