Fig. 3. Nine genes were identified as commonly regulated genes through RNA sequence analysis. (A) Two siSETDB1 were prepared and transfected into A549 cells. SETDB1 protein level was downregulated by siSETDB1 transfection. (B) Up-regulated genes were analyzed after doxorubicin, taxol, and siSETDB1 treatment. (C) Nine genes were identified as common target genes for doxorubicin, taxol, and SETDB1. Among them, FosB gene expression showed the highest change. (D) RT-PCR analysis was performed to check the expression of FosB and EGR2. (E) Western blot showed that doxorubicin treatment increased the FosB gene expression in a dose- or time-dependent manner. (F) Treatment of taxol or etoposide increased the FosB gene expression in a dose-dependent manner.