Figure 5.

UCP2 RNA interference results in a significant increase in ACE mRNA. HUVECs were transfected with two different siRNA against UCP2 (siRNA 1 and siRNA 2) and total RNA isolated at 30 and 48 h. Quantitative RT‐PCR was performed on an ABI prism 7900HT. UCP2 RNA interference resulted in a significant decrease between threefold and sixfold in UCP2 mRNA expression at 30 and 48 h associated with a significant increase in ACE mRNA (1.2‐ to 1.4‐fold at 30 h and 1.8‐fold at 48 h). (Experiments were repeated a minimum of five times; data are mean ± SD.) †P < 0.0001. *P < 0.001.