Skip to main content
. 2015 Dec 10;6(6):471–478. doi: 10.1080/19491034.2015.1126028

Figure 4.

Figure 4.

Effect of IPTG on luciferase expression in Seh1-LacI-YFP tethered lines.A) RT-qPCR of total RNA extracted from tethered and non-tethered lines 20h after mock or 100 mM IPTG treatment. RNA levels of luciferase were similar in mock-treated (1.16± 0.47) and IPTG-treated (1.19 ± 0.32) LacO:Luc seedlings (n=3 , p=0 .45). In the LacO:Luc+ Seh1-LacI-YFP tethered line, luciferase RNA levels decreased after IPTG treatment (1.12 ± 0.22) compared to the mock treatment (2.10 ± 0.49) n=3 , p=0 .037. Average of biological replicates ± sem. B) Anti-Luc antibody detected a specific luciferase band and a smaller, non-specific band in total protein extract from tethered and non-tethered lines with mock and IPTG treatment. The average band intensity of the luciferase and the internal band (as control) were measured as an approximation of protein quantity and show that protein levels of luciferase increase significantly in the tethered line and decrease significantly after IPTG treatment. Data from one blot representative of 3 biological repeats.These changes were only seen for the luciferase band and not the internal control bands.