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. 2015 Dec 8;6(6):479–489. doi: 10.1080/19491034.2015.1128608

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© 2015 The Author(s). Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License http://creativecommons.org/licenses/by-nc/3.0/, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 6. — Ca²⁺ imaging in cells expressing SP-GFP-SUN1-C. (A) Effect of SP-GFP-SUN1-C polypeptide interfering with Nesprin localization in a dominant manner. HaCaT cells were transfected either with control SP-GFP plasmid or SP-GFP-SUN1-C plasmid. Immunofluorescence analysis was performed using pAbK1 Nesprin-2 antibodies and DAPI. (B) Representative image of live transfected cells loaded with Rhod-2. (C) Quantitative comparison of fluorescence measurements in the transfected cell lines (F: corrected Rhod-2 fluorescence intensity): The values represent mean and SD of 3 independent experiments (p value, 0.0021). 50 cells were analyzed.