Table 1.
PG-localized and related FIBRILLIN proteins that show reported and (mostly) confirmed p-peptides
| Protein i.d.a | Protein name | Locationb | Abundance rank in isolated PGc | Module in coexpresion networkd | Reported p-peptidese | p-Site residue number (low confidence p-sites)f | Protein length (predicted N-term (exp. N-term))g | Motif subtype (based on van Wijk et al. 2014) |
|---|---|---|---|---|---|---|---|---|
| AT4G0402 | FBN1A | Core PG | 1 | Not in network | VFApSpSpSpTVpSVADK | S74, S75, S76, T77, S79 | 318; A56 (S41, A56, T57) | SxpS, pSxS |
| AT4G0402 | FBN1A | Core PG | 1 | Not in network | ApTDIDDEWGQDGVER | T57 | 318; A56 (S41, A56, T57) | TD |
| AT4G2224 | FBN1B | Core PG | 3 | Not in network | ApTDTGEIGSALLAAEEAIEDVEETERLKR | T61 | 310; A60 (A60) | TD |
| AT4G2224 | FBN1B | Core PG | 3 | Not in network | FAGPLGpTNSISTNAK | T188 | 310; A60 (A60) | — |
| AT2G3549 | FBN2 | Core PG | 4 | III (ABC1K9) | pSGPELEESGTR | S105 | 376; S54 (S54) | SG, SxP |
| AT2G3549 | FBN2 | Core PG | 4 | III (ABC1K9) | SGPELEESGpTR | T114 | 376; S54 (S54) | — |
| AT3G2340 | FBN4 | Core PG | 2 | III (ABC1K9) | LLSVVpSGLNR | S96 | 284; S73 (A54, S57, S58) | SG |
| AT3G2340 | FBN4 | Core PG | 2 | III (ABC1K9) | GLVApSVDDLER | S105 | 284; S73 (A54, S57, S58) | — |
| AT3G2340 | FBN4 | Core PG | 2 | III (ABC1K9) | LLYSSAFpSSR | S148 | 284; S73 (A54, S57, S58) | — |
| AT3G2340 | FBN4 | Core PG | 2 | III (ABC1K9) | pSLGGSRPGLPTGR | S151 | 284; S73 (A54, S57, S58) | xSx, RS |
| AT3G2340 | FBN4 | Core PG | 2 | III (ABC1K9) | SLGGpSRPGLPTGR | S155 | 284; S73 (A54, S57, S58) | GS |
| AT2G4213 | FBN7B | Core PG | 13 | IV | IETPSSTVVETIEYDpS (C-terminus) | S299 | 299; A49 (A49, V51) | DS |
| AT1G3222 | FLAVIN REDUCATASE RELATED 1 | Core PG | 14 | Connected to UBIE1 | SCVKCTYAEAGLpSSASWpSAPIDIVADVK | (S46), S51 | 296; A58 (T39) | SxP, SxpS |
| AT1G3222 | FLAVIN REDUCATASE RELATED 1 | Core PG | 14 | Connected to UBIE1 | SCVKCTYAEAGLSpSASWpSAPIDIVADVK | (S47), S51 | 296; A58 (T39) | SxP, SxpS, pSxS |
| AT2G3446 | FLAVIN REDUCATASE RELATED 2 | Core PG | 21 | II (ABC1K1, 3 ,6) | pSYKDLFASVK | S269 | 280; A52 (M35) | SxxD/E |
| AT1G0669 | NAD(P)-ALDO/KETO REDUCTASE | Core PG | 20 | II (ABC1K1, 3 ,6) | LGGpSDLKVTK | (S54) | 377; A32 (A32, V33) | GS, xSDx |
| AT4G1320 | UNKOWN 1 | Core PG | 15 | III (ABC1K9) | TIMVDVEESSSSpSDED | S182 | 185; C57 | SDx[D/E] |
| AT4G3277 | VTE1 (cTP) | Core PG | 10 | Not connected | SISRVpSApSIpSpTPNSETDK (part of cTP) | S47, S49 (S51, T52) | 488; V99 (S49) | pSxS, SxpS, TP |
| AT5G4265 | AOS | Recruited to PG | Not in network | ASGpSETPDLTVATR | S36 | 518; A33 (S34) | SxpS, GS | |
| AT5G4265 | AOS | Recruited to PG | Not in network | ApSGSETPDLTVATR | S34 | 518; A33 (S34) | pSxS, SG | |
| AT3G4514 | LOX2 | Recruited to PG | Not in network | EFYEpSPEK | (S787) | 896; R56 (C50) | SP | |
| AT3G2607 | FBN3A | Thylakoid | Not in network | GATApSPDDQLR | S92 | 242; V51 | SP | |
| AT4G0003 | FBN9 | Plastid | Not in network | SSITTDDSLSApTWR | (T87) | 212; C26 | — | |
Rows marked in grey indicate lower confidence p-sites. For details, see Supplementary Table S1.
a For proteins with more than one model, we found that model 1 was the best model or that there was no difference in protein sequence with other models. Only in the case of AT2G4213 did we find that model 4 was the most relevant and likely correct model.
b Curated location based on all available information.
c Relative abundance of PG proteins based on label free spectral counting from Lundquist et al. (2012).
d Assignment to mRNA based co-expression modules as published in Lundquist et al. (2012). Co-expression module I contains ABC1K7, module II contains ABC1K1,3,6, module III contains ABC1K9 and module IV has no kinases. ABC1K5 connects to both module II and III.
e Reported p-peptides in databases or publications. All pS or pT sites are shown in bold.
f p-Site residue number.
g Protein length and predicted or experimental N-terminus. For data see PPDB. Experimental N-termini are based on N-terminal acetylated residues or dimethyl-labelled residues from TAILS experiments (see Rowland et al., 2015). In some cases more than one N-terminus is observed in dimethyl-labelling experiments. Bold indicates the most likely/most frequent experimentally observed N-terminus (based on data in Rowland et al. (2015)).