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. 2016 Jun 21;57(3):312–317. doi: 10.1093/jrr/rrw001

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© The Author 2016. Published by Oxford University Press on behalf of The Japan Radiation Research Society and Japanese Society for Radiation Oncology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Fig. 3. — Intracellular ROS levels (A), and DSBs determined by the neutral comet assay (B) and 53BP1/γH2AX staining (C to E) in control PCCL3 cells and cells pretreated with 20 mM NAC and/or irradiated or exposed with H₂O₂. The data were obtained 0.5 h (the comet assay and γH2AX) or 1.5 h (53BP1) after irradiation or 10 min after addition of H₂O₂. *P < 0.05; **P < 0.01.