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. 2016 May 17;64(7):1252–1264. doi: 10.1002/glia.22997

Figure 4.

Figure 4

Electrical stimulation mediates GLT‐1 transporter surface mobility at activated synapses. A: Representative images of hippocampal neurons transfected with SyGCaMP5 (green) at 7‐10 DIV, cocultured with astrocytes pretransfected with GLT‐1‐V5 (Red). Cocultures were maintained for 3‐4 DIV and imaged using an image splitter. Scale bar, 10 μm. B: Region boxed in (A) and representative single trajectories shown before stimulation (top panel), and during (bottom panel, 10 Hz for 10 s, 100 PA). Scale bar, 2 μm. C: Time course of SyGCaMP5 fluorescence F/F0 (6 synapses). Background signal at each corresponding time point was subtracted from the SyGCaMP5 signal before normalizing to the first 10 frames (F/F0). D: Percentage of change in GLT‐1 surface diffusion significantly increased during stimulation compared to before (n = 6 trajectories, P = 0.04, Mann‐Whitney test).