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. 2016 Jun 14;34(28):3252–3259. doi: 10.1016/j.vaccine.2016.05.007

Fig. 3.

Fig. 3

Induction of antibody following immunization with Pvs25-FhCMB. The ability of different immunization regimes to generate Pvs25-specific antibody responses was tested by ELISA against recombinant Pvs25-FhCMB and IFA against Pvs25DR3 ookinetes. (a) Pre-boost and end-point titers of anti-Pvs25-FhCMB in serum. Bars show mean titers from five mice. Light grey = pre-boost, dark grey = post-boost. Pre-immune serum did not recognize recombinant Pvs25-FhCMB. Error bars represent SEM. (b) End-point titers of anti-r-Pvs25 serum. Bars show mean titers from five mice. Error bars represent SEM. (c) IFA against Pvs25DR3 ookinetes. Ability of generated serum to recognize native Pvs25 on the surface of transgenic Pvs25DR3 ookinetes was assessed by immunofluorescence on fixed, non-permeabilized parasites probed with anti-serum from each regime. To control for non Pvs25-specific signal, IFA was performed against WT 2.34 ookinetes. Each panel shows an overlay of anti-Pvs25 signal (green) and DNA labeled with DAPI (blue). IFA with non-Pvs25 derived (control) serum from regimes 5−8 resulted in no significant staining.