Fig 4. Functional analysis of the 5’-flanking region of the human TBX15 gene.

(A) Representation of the different luciferase reporter constructs used in the analysis, with predicted NF-κB binding sites (kBS1, kBS2 and kBS3). (B) The TBX15 promoter and its 5’-flanking region activity measured by luciferase in TNF-α treated and untreated cells. Vector expressing the Renilla luciferase was co-transfected as an internal reference to correct the transfection efficiency. (C) Luciferase analysis of the different reporter constructions. Data was expressed as fold change referred to untreated cells as 1. At least three independent experiments were performed with duplicates. Bars represent the mean±SD. * indicates p-value < 0.05 and ** p-value < 0.01.