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. 2016 Jun 21;11(6):e0157842. doi: 10.1371/journal.pone.0157842

Fig 2. Deoxycholate/Sephacryl S-200-based gel sieving of EtOHp to isolate capsule.

Fig 2

A) Results of gel sieving of the EtOHp. Recoveries of loaded cpm were typically ~100%. Fractions used for subsequent SDS-PAGE analysis are numbered. B) Capsule (11B7) immunoblot, C) O-antigen (FB11) immunoblot, and (D) autoradiogram, respectively of the indicated fractions. Data shown are representative of at least 4 independent experiments. E) EtOHp and capsule were subjected to Bligh-Dyer extraction and the partitioning of cpm is indicated. Numbers do not add up to 100% due to losses. F) O-antigen immunoblot and autoradiogram of EtOHp and of the extracted lipid fraction following Bligh-Dyer extraction of EtOHp.