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. 2016 Jun 21;11(6):e0157842. doi: 10.1371/journal.pone.0157842

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© 2016 Barker et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 4 — A) HPTLC of lipids recovered after mild acid hydrolysis of the indicated preparations. The solvent system used was CHCl₃:Pyridine:88% Formic Acid:MeOH:H₂O (54:46:16:0:5 v/v). The arrowhead indicates the dominant species in free lipid A. Highly enriched free lipid A was derived via Bligh-Dyer extraction of the EtOHp (see Fig 2F). B) HPLC analysis of [¹⁴C] fatty acids derived from free lipid A and lipid released by mild acid treatment of HMW O-Ag. C) The four major peaks in B were quantified and expressed as the % of total recovered cpm.