Fig 2. NER dual incision at adducts in the NarI sequence in the human NER system.
Plasmids containing site-specific mono-FAAF (lanes G1, G2, G3) or di-FAAF adducts (lanes G1G2, G2G3, G1G3), were incubated with HeLa whole-cell extracts. Detection of excision products was monitored by 3’-end-labeling using a complementary oligonucleotide containing a 5’-GGGG base overhang. The reaction products were resolved on a 12% denaturing polyacrylamide gel run under constant current. The range of excision products is indicated on the left of the gel.