Fig 1. cln3 is expressed in the developing WT zebrafish.

A-N) In situ hybridisation for cln3 (antisense) shows its expression from the one cell stage through to 4 dpf. From 1 dpf expression is increasingly restricted to the central nervous system. At 2 dpf cln3 expression is particularly strong in the mid-hindbrain boundary (arrow) where the cerebellum is developing (G, H, M, N). (A’-L’) Experiments conducted using a cln3 sense probe (sense) revealed the specificity of the antisense signal. Abbreviations: mhb, mid-hindbrain boundary.