Fig 4. Immunogenicity of the hexon chimeric rAd3H14 vector expressing EGFP in mice with anti-Ad3 immunity.

Naive mice or mice with anti-Ad3 immunity were immunized intramuscularly with 10¹⁰ VPs of rAd3EGFP or rAd3H14 vector. EGFP-specific humoral immune responses were detected by indirect-ELISA with recombinant GFP protein as the capture antigen at 2 weeks after immunization. Each experiment was repeated independently at least three times. Data are shown as means ± SEM. * P<0.05.