Fig 2. Physical localization assay of the three TaGID2s.
(A) Chromosome location of the three TaGID2s, the genomic DNA of a set of Chinese Spring nulli-tetrasomic lines was amplified using the homolog-specific primers of TaGID2-1, TaGID2-2, and TaGID2-3, with ddH2O as a control. (B) Chromosomal bin location of the three TaGID2s, products amplified from 11 deletion lines of Chinese Spring using the homolog-specific primers of TaGID2-1, TaGID2-2, and TaGID2-3 were detected by 1.5% agarose gel, with ddH2O as a control. (C) Position of the three TaGID2s in chromosomes 3A, 3B and 3D, the names and breakpoints of the deletion lines are shown on the left, and the chromosome bins and TaGID2 genes are shown on the right.