Fig 5. Interaction between TaGID2 and RHT-1 in yeast cells.

(A) TaGID2s interact with RHT-1 in yeast cells, TaGID2-A1, TaGID2-B1, and TaGID2-D1 were used as baits, and RHT-A1a, RHT-B1a, and RHT-D1a were used as preys, the AD and BK constructs were co-transformed into the yeast strain AH109, the transformants were grown on the SD/-Leu-Trp media (control) and the SD/-Leu-Trp-His-Ade media containing 20 μg/mL X-α-Gal with or without 100 μM GA₃, all experiments were conducted three times, and five clones were used each time. (B) TaGID2s interact with allelic variations of RHT-1 in the LHRII motif, SLN1 and RHT-1s were used as preys, TaGID2-A1, TaGID2-B1, and TaGID2-D1 were used as baits from left to right in each block, and the AD and BK constructs were co-transformed into the yeast strain AH109, the transformants were grown on the SD/-Leu-Trp media (control) and the SD/-Leu-Trp-His-Ade media with or without 100 μM GA₃, all experiments were conducted three times, and five clones were used each time.