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. 2016 Jun 21;11(6):e0157642. doi: 10.1371/journal.pone.0157642

Table 1. Polymerase chain reaction primers used in this study.

Primer Forward primer sequence Reverse primer sequence
Contig1.GID2.F/R 5′-TGTCCATCTCTCCTCGTCAGATCCATC-3′ 5′-CGGTGCGCTGGACTGGTTGA-3′
TSK1.F/R 5′-CGCGACTAGAGTTTCCTCGCTAGGG-3′ 5′-ACGATTAAGATTCAGTTTGACAAGT-3′
TaGID2-1LF/1LR 5′-ACGACGAGCACAGGTGAGCAC-3′ 5′-CAACAACCAGTATGGATCATGAA-3′
TaGID2-2LF/2LR 5′-AGCACGAGCACAGGTGAGTAT-3′ 5′-CCTTGTTCTTTGGCCAGCTT-3′
TaGID2-3LF/3LR 5′-AGCACGAGCACAGGTGAGTAT-3′ 5′-TGGCCAAAAGAACAGAGCATA-3′
TaGID2-A-HindIII.F/BamHI.R 5′-AAGCTT ATGAAGTGCCCTTCCGATTCCTC-3′ 5′-GGATCC CTGGGACGGCGAGGGAG-3′
TaGID2-B-HindIII.F/BamHI.R 5′-AAGCTT ATGAAGCGCCCTTCCGGTT-3′ 5′-GGATCC CTGGGATGGCGAGGGAG-3′
TaGID2-D-HindIII.F/BamHI.R 5′-AAGCTT ATGAAGTGCCCTTCCGATTCCTC-3′ 5′-GGATCC CTGGGACGGCGCGGGAG-3′
TaGID2-A-attB.F/R 5′-GGGGACAAGTTTGTACAAAAAAGCAGGCTGC ATGAAGTGCCCTTCCGATTCCTC-3′ 5′-GGGGACCACTTTGTACAAGAAAGCTGGGTC CTGGGACGGCGAGGGAG-3′
TaGID2-B-attB.F/R 5′-GGGGACAAGTTTGTACAAAAAAGCAGGCTGC ATGAAGCGCCCTTCCGGTT-3′ 5′-GGGGACCACTTTGTACAAGAAAGCTGGGTC CTGGGATGGCGAGGGAG-3′
TaGID2-D-attB.F/R 5′-GGGGACAAGTTTGTACAAAAAAGCAGGCTGC ATGAAGTGCCCTTCCGATTCCTC-3′ 5′-GGGGACCACTTTGTACAAGAAAGCTGGGTC CTGGGACGGCGCGGGAG-3′
TaGID2-A-EF/ER 5′-GCATCCTCGTCCTCACAG-3′ 5′-GAAAGAGAGCACGACGTCG-3′
TaGID2-B-EF/ER 5′-TCACAGCCTCCACCGGC-3′ 5′-GAACGGGAGCACGACGG-3′
TaGID2-D-EF/ER 5′-ACAGCCTCCGCCGCAGCA-3′ 5′-CCTCCTCCTCCCCCGTTC-3′
Ta4045-EF/ER 5'-CCTGCCCCGTACAACCTTGAG-3' 5'-CACCGTTGCGATAGTCCTGAAAC-3'
TSK1-NdeI.F/EcoRI.R 5′-CATATG ATGGCGGCCGCGGGAGAC-3′ 5′-GAATTC CTACTCAAAGGCCCACTGGTTCTC-3′
TaGID2-A1-NdeI.F/EcoRI.R 5′-CATATG ATGAAGTGCCCTTCCGATTCCTC-3′ 5′-GAATTC TCACTGGGACGGCGAGGGAG-3′
TaGID2-B1-NdeI.F/EcoRI.R 5′-CATATG ATGAAGCGCCCTTCCGGTT-3′ 5′-GAATTC TCACTGGGATGGCGAGGGAG-3′
TaGID2-D1-NdeI.F/EcoRI.R 5′-CATATG ATGAAGTGCCCTTCCGATTCCTC-3′ 5′-GAATTC TCACTGGGACGGCGCGGGAG-3′
Rht-A1-NdeI.F/EcoRI.R 5′-CATATG AAGCGCGAGTACCAGGAC-3′ 5′-GAATTC TCAAAACTCGCGATCACG-3′
Rht-B1-NdeI.F/EcoRI.R 5′-CATATG AAGCGCGAGTACCAGGACG-3′ 5′-GAATTC TCAAAACTCGCGGTCACGG-3′
Rht-D1-NdeI.F/EcoRI.R 5′-CATATG AAGCGGGAGTACCAGGACG-3′ 5′-GAATTC TCAAAACTCGCGAGATCACG-3′
TaGID1A-EcoRI.F/BamHI.R 5′- GAATTC ATGGCCGGCAGCGACGAG -3′ 5′- GGATCC CTACAGGAGGTTAGCTCGGACGA -3′
TaGID2A-NotI.F/BglII.R 5′- GCGGCCGC ATGAAGTGCCCTTCCGATTCCTC -3′ 5′- AGATCT TCACTGGGACGGCGAGGGAG -3′