Table 1. P. falciparum gene names (abbreviation), ID numbers and primers used for amplification of CDKs and cyclins.
The seryl-tRNA synthetase gene was used as a positive control.
| Gene name (abbreviation) Gene ID | Sequences 5’ to 3’ |
|---|---|
| Pf seryl-tRNA synthetase (Pfsars) Pf3D7_0717700 | Pfsars-f AAGTAGCAGGTCACGTG |
| Pfsars-r CGGCACATTCTTCCATA | |
| Pf MO15-related protein kinase (Pfmrk) Pf3D7_1014400 | Pfmrk-f TGTGGAGTTTTGGTTGTATTTTT |
| Pfmrk-r TTCTGGCCAATTATTTTCGTTAG | |
| Pf Protein Kinase 5 (pfpk5) Pf3D7_1356900 | Pfpk5-f TCGAGCACCAGATGTTTTAATGGGA |
| Pfpk5-r TGGTCTGCTTCAGATACCCCTGGA | |
| Pf Protein Kinase 6 (pfpk6) Pf3D7_1337100 | Pfpk6-f AGTGACCACATGACACCTACTGT |
| Pfpk6-r CCATGAATAAACAACCCAAACTCCA | |
| Pf cdc2 related protein kinase 1 (pfcrk1) Pf3D7_0417800 | Pfcrk1-f CGTTCACACTTCCCAAATATAGCCA |
| Pfcrk1-r AGGATGGTTTAATGCTTCCTGTGCT | |
| Pf cdc2 related protein kinase 3 (pfcrk3) Pf3D7_0415300 | Pfcrk3-f ACGGAAACCACAAATATGGAGCAGA |
| Pfcrk3-r TCCCTATCACTGTAATCCGTGACCG | |
| Pf cdc2 related protein kinase 4 (pfcrk4) Pf3D7_0317200 | Pfcrk4-f AAAGAAGAGCATGTTGAAAAGGGCA |
| Pfcrk4-r TTCGTCATGCCACTTTTATTGGTGT | |
| Pf cyclin 1 (pfcyc1) Pf3D7_1463700 | Pfcyc1-f CGCAAATGCGGATATGTTTATCT |
| Pfcyc1-r ACCGATTTGTCTGGTCGTATTT | |
| Pf cyclin 2 (pfcyc2) Pf3D7_1227500 | Pfcyc2-f AAGTCGGTGGACGAGACAGC |
| Pfcyc2-r TGGGGGACAAATCATCTACTTCGT | |
| Pf cyclin 3 (pfcyc3) Pf3D7_0518400 | Pfcyc3-f AGGGAGAAGTACCAAGAACCGA |
| Pfcyc3-r TCAGGAACTTTAGAAGCATGAAAAC | |
| Pf cyclin 4 (pfcyc4) Pf3D7_1304700 | Pfcyc4-f ACATCCTCATTCATTTCTTTTACCA |
| Pfcyc4-r TCCCCAAGACATTTGTGCTA |