Table 1. MIP genes in P. Virgatum.
| Gene Name | Phytozome accessions | Genomic Location | PPL(aa) | Maximum Identity with other MIP (%)x | PSCLy | Ka/Ks value |
|---|---|---|---|---|---|---|
| PvPIP1;1 | Pavir.Gb01084.2 | Chr07b: 13931659–13933701 | 288 | XP_002454508(98)a | PLAS, CHLO | 0.095 |
| PvPIP1;2 | Pavir.J11645.1 | contig141014: 535–2653 | 288 | AAO86706(97)b | PLAS, CHLO | 0.380 |
| PvPIP1;3 | Pavir.Gb01084.3 | Chr07b: 13931659–13933701 | 277 | AAO86706(97)b | PLAS | 0.669 |
| PvPIP1;4 | Pavir.Aa00868.1 | Chr01a: 10299092–10302435 | 289 | XP_004953388(99)c | PLAS, CHLO | 0 |
| PvPIP1;5 | Pavir.J37677.1 | contig69730: 133–3636 | 289 | XP_004953388(99)c | PLAS, CHLO | 0.116 |
| PvPIP1;6 | Pavir.Aa00075.1 | Chr01a: 810607–812141 | 288 | NP_001105131(98)b | PLAS, CHLO | 0.052 |
| PvPIP1;7 | Pavir.Ab03380.1 | Chr01b: 55703303–55704564 | 288 | NP_001105131(99)b | PLAS, CHLO | 0.023 |
| PvPIP2;1 | Pavir.Ab02356.1 | Chr01b: 44317427–44320839 | 288 | NP_001105026(98)b | PLAS, CHLO | 0.307 |
| PvPIP2;2 | Pavir.Ab02356.2 | Chr01b: 44317427–44320300 | 264 | ACG33001(98)b | PLAS | 0.569 |
| PvPIP2;3 | Pavir.Bb01320.1 | Chr02b: 27409376–27413184 | 363 | NP_001105024(98)b | PLAS | 0.103 |
| PvPIP2;4 | Pavir.Ga01149.1 | Chr07a: 14124713–14126981 | 266 | XP_004976254(96)c | PLAS | 0.463 |
| PvPIP2;5 | Pavir.Gb00671.1 | Chr07b: 7857237–7859623 | 277 | XP_004976254(99)c | PLAS | 0.505 |
| PvPIP2;6 | Pavir.Ba02483.2 | Chr02a: 37691323–37694961 | 290 | XP_002461930(99)a | PLAS, CHLO | 0.186 |
| PvPIP2;7 | Pavir.Bb01320.2 | Chr02b: 27409376–27413188 | 290 | XP_002461930(99)c | PLAS, CHLO | 0.688 |
| PvPIP2;8 | Pavir.Bb01841.1 | Chr02b: 46595867–46597386 | 286 | XP_004956116(97)c | PLAS, CHLO | 0.331 |
| PvPIP2;9 | Pavir.Ba02478.1 | Chr02a: 37576388–37578287 | 286 | XP_004956116(98)c | PLAS, CHLO | 0.201 |
| PvPIP2;10 | Pavir.Ib04237.1 | Chr09b: 67322496–67323750 | 276 | XP_002489214(90)a | PLAS | 0.111 |
| PvPIP2;11 | Pavir.Ia02751.1 | Chr09a: 54199846–54200694 | 282 | XP_002489214(89)a | PLAS | 0.238 |
| PvPIP2;12 | Pavir.Ib03181.1 | Chr09b: 51605207–51606895 | 294 | XP_004986496(84)c | PLAS | 0.476 |
| PvPIP2;13 | Pavir.Ba01199.1 | Chr02a: 15220158–15221702 | 284 | XP_004957505(85)c | PLAS | 1.323 |
| PvPIP2;14 | Pavir.J11644.1 | contig140997: 365–1545 | 287 | XP_004957505(83)c | PLAS | 0.327 |
| PvTIP1;1 | Pavir.Ia04869.1 | Chr09a: 86386535–86388928 | 250 | P50156(96)d | VACU | 0 |
| PvTIP1;2 | Pavir.Ib00275.1 | Chr09b: 2982020–2984273 | 250 | P50156(96)d | PLAS | 0.267 |
| PvTIP1;3 | Pavir.Ea04152.1 | Chr05a: 63625246–63626300 | 252 | XP_004971442(92)c | VACU | 0 |
| PvTIP1;4 | Pavir.Ea04152.2 | Chr05a: 63625246–63626533 | 252 | XP_004971442(91)c | PLAS | 0 |
| PvTIP2;1 | Pavir.Gb01125.1 | Chr07b: 14244344–14245618 | 248 | XP_004976439(98)c | PLAS | 0.035 |
| PvTIP2;2 | Pavir.Ga01087.1 | Chr07a: 12722976–12724266 | 248 | XP_004976439(98)c | PLAS | 0.076 |
| PvTIP2;3 | Pavir.J30578.1 | contig357494: 1–1165 | 249 | XP_004953349(98)c | PLAS | 0.203 |
| PvTIP2;4 | Pavir.Da01714.1 | Chr04a: 37554270–37555738 | 248 | XP_002438430(97)a | PLAS | 0.212 |
| PvTIP2;5 | Pavir.Db01217.1 | Chr04b: 23834192–23835703 | 248 | XP_002438430(97)a | PLAS | 0.063 |
| PvTIP3;1 | Pavir.Ia01749.1 | Chr09a: 21354884–21356273 | 263 | NP_001105032(95)b | MITO | 0.271 |
| PvTIP3;2 | Pavir.Ib03520.1 | Chr09b: 57398634–57399928 | 264 | NP_001105032(95)b | MITO | 0.033 |
| PvTIP3;3 | Pavir.Ga00845.1 | Chr07a: 10052517–10054500 | 273 | XP_002446824(88)a | CHLO | 0.456 |
| PvTIP4;1 | Pavir.Ea00003.1 | Chr05a: 159662–160973 | 250 | XP_004967395(94)c | VACU | 0.682 |
| PvTIP4;2 | Pavir.J30482.1 | contig355910: 206–1208 | 256 | XP_004967395(91)c | CYTO | 0.102 |
| PvTIP4;3 | Pavir.J20433.1 | contig222165: 1187–2025 | 239 | XP_004967395(88)c | VACU | 0.819 |
| PvTIP4;4 | Pavir.Eb00023.1 | Chr05b: 514689–516054 | 259 | XP_004967394(92)c | CYTO | 0.521 |
| PvTIP4;5 | Pavir.Cb01832.1 | Chr03b: 43764796–43767586 | 347 | XP_004960662(93)c | CHLO | 0.154 |
| PvTIP4;6 | Pavir.Ca00461.1 | Chr03a: 5397927–5400491 | 318 | XP_004960662(91)c | CYTO | 0.172 |
| PvTIP5;1 | Pavir.Gb01126.1 | Chr07b: 14245888–14247100 | 270 | XP_004978166(82)c | CHLO | 0.187 |
| PvTIP5;2 | Pavir.Ga01088.1 | Chr07a: 12724501–12725804 | 266 | XP_004978166(78)c | CHLO | 0.522 |
| PvNIP1;1 | Pavir.Cb01700.1 | Chr03b: 42769884–42772044 | 280 | XP_004960601(95)c | PLAS | 0.289 |
| PvNIP1;2 | Pavir.J36379.1 | contig59709: 2228–4657 | 277 | XP_004960601(93)c | PLAS | 0.288 |
| PvNIP1;3 | Pavir.Eb00236.3 | Chr05b: 3774233–3776780 | 290 | XP_002454982 (89)a | PLAS | 0.137 |
| PvNIP1;4 | Pavir.Ea00222.1 | Chr05a: 2686340–2692046 | 287 | XP_002454982(89)a | PLAS | 0.113 |
| PvNIP1;5 | Pavir.Ab01231.1 | Chr01b: 18627382–18630325 | 280 | XP_004951368(93)c | PLAS | 0.436 |
| PvNIP1;6 | Pavir.Db00851.1 | Chr04b: 11796860–11798059 | 322 | XP_004967095(73)c | PLAS | 0.328 |
| PvNIP1;7 | Pavir.Da00802.1 | Chr04a: 12785576–12787025 | 287 | XP_004967095(83)c | PLAS | 0.327 |
| PvNIP2;1 | Pavir.Ab02995.1 | Chr01b: 52353467–52357364 | 296 | XP_004953867(97)c | E.R | 0.116 |
| PvNIP2;2 | Pavir.Aa00406.1 | Chr01a: 4613561–4619572 | 313 | XP_004953867(76)c | CHLO | 0.082 |
| PvNIP2;3 | Pavir.Db01588.1 | Chr04b: 35916639–35920941 | 295 | XP_004965042(97)c | PLAS | 0.423 |
| PvNIP2;4 | Pavir.Da01156.1 | Chr04a: 22506078–22510755 | 296 | XP_004965042(97)c | PLAS | 0.049 |
| PvNIP3;1 | Pavir.J11993.1 | contig143579: 44–1260 | 286 | XP_004974441(80)c | VACU | 0.460 |
| PvNIP3;2 | Pavir.J04994.1 | contig07346: 8718–11701 | 292 | XP_004974441(81)c | PLAS | 0.255 |
| PvNIP3;3 | Pavir.Fb00252.1 | Chr06b: 4491634–4492686 | 288 | XP_004974441(84)c | CHLO | 0.672 |
| PvNIP3;4 | Pavir.Fa01950.2 | Chr06a: 45025793–45028094 | 330 | XP_004974441(82)c | CYTO | 0.153 |
| PvNIP3;5 | Pavir.Fa01948.1 | Chr06a: 45006164–45007342 | 298 | XP_004974438(87)c | CYTO | 0.598 |
| PvNIP3;6 | Pavir.Fa01949.1 | Chr06a: 45022689–45023983 | 278 | XP_004974438(93)c | PLAS | 0.560 |
| PvNIP3;7 | Pavir.J17719.1 | contig194795: 1290–2237 | 291 | XP_004974439(81)c | PLAS | 0.315 |
| PvNIP3;8 | Pavir.J35034.1 | contig50657: 4142–5117 | 295 | XP_004974439(80)c | CYTO | 0.398 |
| PvNIP3;9 | Pavir.Ib03684.1 | Chr09b: 59774269–59780622 | 301 | XP_004982621(98)c | PLAS | 0.594 |
| PvNIP3;10 | Pavir.Ia01421.1 | Chr09a: 15383290–15385609 | 281 | XP_002464380(87)a | CHLO | 0.561 |
| PvNIP4;1 | Pavir.Ea00764.2 | Chr05a: 10523338–10525337 | 310 | XP_004971599(86)c | PLAS | 1.429 |
| PvNIP4;2 | Pavir.Ea00764.3 | Chr05a: 10523338–10525337 | 308 | XP_004971599(85)c | PLAS | 1.309 |
| PvSIP1;1 | Pavir.J16825.1 | contig18611: 427–3959 | 243 | XP_004962139(95)c | PLAS | 0.107 |
| PvSIP1;2 | Pavir.J10110.1 | contig12910: 934–4304 | 241 | XP_004962139(95)c | PLAS | 0.060 |
| PvSIP2;1 | Pavir.Ia03463.1 | Chr09a: 68891317–68893364 | 242 | XP_004984561(97)c | NUCL | 0.135 |
| PvSIP2;2 | Pavir.J37350.1 | contig67361: 886–3091 | 242 | XP_004984561(97)c | PLAS | 0.164 |
Where, Ka and Ks are numbers of non-synonymous and synonymous substitutions per site, respectively. PPL: polypeptide length, aa: amino acid, PSCL: predicted subcellular localization, PLAS: plasma membrane. VACU: vacuolar membrane, CYTO: cytosol, ER: endoplasmic reticulum, MITO: mitochondrion, NUCL: Nucleous and CHLO: chloroplast.
xA gene that shows the highest identity with MIP in other plants by BLASTp. Parenthesis indicates the percentage of identity at the amino acid level.
aSorghum bicolor
bZea mays
cSetaria italica and
dOryza sativa Japonica Group